Alaska House Backtracks On Full Dividend Vote

first_imgFacebook0TwitterEmailPrintFriendly分享JUNEAU, Alaska (AP) — The Alaska House’s populist vote to pay Alaskans the full amount for their oil-wealth fund checks has been undone. Lawmakers rescinded the vote Friday after debate over Alaska Permanent Fund dividends derailed efforts to pass a state operating budget. Monday’s vote for a fully funded dividend, held during a drawn-out budget debate, split the House majority and spurred minority Republicans to seek bigger budget cuts to win over their members. The size of the oil check was cut roughly in half the last two years amid gridlock over Alaska’s budget deficit.center_img A handful of majority members who supported a full dividend Monday backed undoing that vote Friday. Rather than a full payout, estimated at about $2,650, lawmakers voted for a $1,600 dividend this year.last_img read more

Amazon will open a new Denver office add 400 jobs

first_img Share your voice Amazon,I’m shocked — shocked! — to learn that stores are turning Labor Day into an excuse to sell stuff. Wait — no, I’m not. As much as I respect the original intent of the holiday (which became official back in 1894), to most of us, it’s just a bonus day off — one that’s blissfully tacked onto a weekend. So, yeah, stores; go ahead, run your sales. I’m listening. Perhaps unsurprisingly, Labor Day doesn’t bring out bargains to compete with the likes of Black Friday (which will be here before you know it), but there are definitely some sales worth your time.For example:We’ve rounded up the best Labor Day mattress deals.We’ve also gathered the best Labor Day laptop deals at Best Buy.The 2019 Vizio P Series Quantum is back under $999.Be sure to check out Amazon’s roughly three dozen Labor Day deals on TVs and audio. Google Express is having a big sale as well, one that includes deals on game consoles, AirPods, iPhones, laptops and more.Below I’ve rounded up a handful of individual items I consider to be the cream of the crop, followed by a handy reference guide to other Labor Day sales. Keep in mind, of course, that products may sell out at any time, even if the sale itself is still running. Note that CNET may get a share of revenue from the sale of the products featured on this page. Recently updated to include digital-photo-frame capabilities, the Lenovo Smart Clock brings Google Assistant goodness to your nightstand. It’s a little smaller than the Amazon Echo Show 5, but also a full $30 less (and tied with Prime Day pricing) during this Best Buy Labor Day sale. 4:00 Though not technically a Labor Day sale, it’s happening during Labor Day sale season — and it’s too good not to share. Nationwide Distributors, via Google Express, has just about the best AirPods deal we’ve seen (when you apply promo code ZBEDWZ at checkout). This is for the second-gen AirPods with the wireless charging case. Can’t imagine these will last long at this price, so if you’re interested, act fast. $210 at Best Buy Angela Lang/CNET Sarah Tew/CNET Read Lenovo Smart Clock review Now playing: Watch this: HP Laptop 15t Value: $520 (save $780) I thought this might be a mistake, but, no, the weirdly named HP Laptop 15t Value is indeed quite the value at this price. Specs include an Intel Core i7 processor, 12GB of RAM, a 256GB solid-state drive and a 15.6-inch display. However, I strongly recommend paying an extra $50 to upgrade that display to FHD (1,920×1,080), because you’re not likely to be happy with the native 1,366×768 resolution. See It The problem with most entry-level laptops: They come with mechanical hard drives. That makes for a mighty slow Windows experience. This Lenovo model features a 128GB solid-state drive, so it should be pretty quick to boot and load software, even with its basic processor. Plus, it has a DVD-burner! That’s not something you see in many modern laptops, especially at this price. Spotify and most other streaming services rely on compressed audio, which robs the listener of full fidelity. Enter Tidal, the only “major” service that delivers lossless audio — meaning at least on par with CD quality, if not better. Want to see (er, hear) the difference for yourself? Grab this excellent extended trial while you can. It’s just $6 for three months, and it’s good for up to six listeners. Lenovo 130-15AST 15.6-inch laptop: $210 (save $90) Free Echo Dot with an Insignia or Toshiba TV (save $50) Read Google Home Hub review See It Turo: Save $30 on any car rental Google Nest Hub: $59 (save $70) $155 at Google Express Read DJI Osmo Action preview Mentioned Above Apple iPhone XS (64GB, space gray) $6 at Tidal $90 at Daily Steals via Google Express Tags $261 at Daily Steals via Google Express $59 at eBay Rylo 7 Best laptops for college students: We’ve got an affordable laptop for every student. Best live TV streaming services: Ditch your cable company but keep the live channels and DVR. $520 at HP Share your voice Chris Monroe/CNET Turo is kind of like Uber meets Airbnb: You borrow someone’s car, but you do all the driving. I’ve used it many times and found it a great alternative to traditional car-rental services — in part because you get to choose exactly the vehicle you want (not just, say, “midsize”) and in part because you can often do pickup and dropoff right outside baggage claim.Between now and Sept. 1, the first 300 people to check out can get $30 off any Turo rental with promo code LDW30. $999 Lenovo Smart Clock: $59.99 (save $20) Apple AirPods with Wireless Charging Case: $155 (save $45) $60 at Best Buy Sarah Tew/CNET Apple iPhone XS Read the AirPods review DJI Osmo Action camera: $261 (save $89) $999 Sarah Tew/CNET See at Amazon Rylo 5.8K 360 Video Camera: $250 (save $250) 1 What’s cooler: A snapshot of a firework exploding in front of you, or full 360-degree video of all the fireworks and all the reactions to seeing them? Oooh, ahhh, indeed. At $250, the compact Rylo dual-lens camera is selling for its lowest price yet. And for an extra $50, you can get the bundle that includes the waterproof housing.This deal runs through Sept. 3; it usually costs $500. $299 at Amazon Read the Rylo camera preview Amazon Other Labor Day sales you should check out Best Buy: In addition to some pretty solid MacBook deals that have been running for about a week already, Best Buy is offering up to 40% off major appliances like washers, dryers and stoves. There are also gift cards available with the purchase of select appliances. See it at Best BuyDell: Through Aug. 28, Dell is offering an extra 12% off various laptops, desktops and electronics. And check back starting Aug. 29 for a big batch of Labor Day doorbusters. See it at DellGlassesUSA: Aug. 29 – Sept. 3 only, you can save 65% on all frames with promo code labor65. See it at GlassesUSALenovo: The tech company is offering a large assortment of deals and doorbusters through Labor Day, with the promise of up to 56% off certain items — including, at this writing, the IdeaPad 730S laptop for $700 (save $300).See it at LenovoLensabl: Want to keep the frames you already love and paid for? Lensabl lets you mail them in for new lenses, based on your prescription. From now through Sept. 2 only, you can save 20% on the blue light-blocking lens option with promo code BLOCKBLUE. See it at LensablSears: Between now and Sept. 7, you can save up to 40% on appliances (plus an additional 10% if you shop online), up to 60% on mattresses, up to 50% on Craftsman products and more. The store is also offering some fairly hefty cashback bonuses. See it at SearsNote: This post was published previously and is continuously updated with new information.CNET’s Cheapskate scours the web for great deals on tech products and much more. For the latest deals and updates, follow the Cheapskate on Facebook and Twitter. Questions about the Cheapskate blog? Find the answers on our FAQ page, and find more great buys on the CNET Deals page. Amazon is expanding its Denver tech hub. Amazon Amazon is opening a new office in downtown Denver and adding 400 new high-tech jobs, the company said Tuesday. This expansion of its Denver hub will more than double Amazon’s tech workforce in the area. The e-commerce giant opened an office in Boulder last fall. The new jobs will be in fields such as software and hardware engineering, cloud computing and advertising.”We’re excited to continue to grow and invest in the Denver area,” said Dave Wood, site lead for Amazon’s Denver tech hub, in a statement. “Our new office will offer our teams the convenience of a downtown location with an array of outdoor adventures just up the road.”The Denver office will be part of a network of 17 development centers across North America, in addition to the company’s main headquarters in Seattle, Washington, and its future second headquarters in Arlington, Virginia. These hubs employ more than 20,000 in total, according to Amazon. In November, Amazon said it’d build two 25,000-employee campuses: one in Long Island City in New York’s Queens borough and another in Arlington. In February, Amazon canceled its plans to build the New York City campus, but said it’d continue with the Virginia location.  Formerly known as the Google Home Hub, Google’s Nest Hub packs a wealth of Google Assistant goodness into a 7-inch screen. At $59, this is within a buck of the best price we’ve seen. It lists for $129 and sells elsewhere in the $89-to-$99 range.This is one item of many available as part of eBay’s Labor Day Sale (which, at this writing, doesn’t specifically mention Labor Day, but that’s how it was pitched to us). An Echo Dot makes a fine match for any Fire edition TV, because you can use the latter to say things like, “Alexa, turn on the TV.” Right now, the 24-inch Insignia Fire TV Edition starts at just $100, while the 32-inch Toshiba Fire TV Editions is on sale for $130. Just add any Fire TV Edition to your cart, then add a third-gen Echo Dot, and presto: The latter is free. See It $999 Turo See at Turo Best Buy Comment Sarah Tew/CNET Tags Sprint $999 Review • iPhone XS review, updated: A few luxury upgrades over the XR JBL Soundgear wearable speaker: $90 (save $160) CNET may get a commission from retail offers. Amazon kills plans for NYC headquarters DJI’s answer to GoPro’s action cameras is rugged little model that’s shockproof, dustproof and waterproof down to 11 meters. It normally runs $350, but this deal drops it to $261 when you apply promo code 19LABOR10 at checkout. TVs Speakers Mobile Accessories Cameras Laptops Automobiles Smart Speakers & Displays Preview • iPhone XS is the new $1,000 iPhone X Use promo code 19LABOR10 to get an unusually good deal on JBL’s interesting hybrid product — not quite headphones, and not quite a traditional speaker, but something you wear like neckphones to listen to music on the go. The Cheapskate Tidal 3-month family subscription: $5.99 (save $54) See it Comments Tech Industry Boost Mobilelast_img read more

Atlanta Illustrator Marcus Williams Brings Diversity to Baltimore ComicCon

first_imgIllustrator Marcus Williams.The annual event known as Baltimore Comic-Con is an opportunity where a cult of artists, creators and writers come together to not be judged. Marcus Williams, an Atlanta, Georgia based illustrator is one of those unique individuals. His work is a representation of a diverse outlook on life.At Baltimore Comic-Con, you get a chance to dress like your favorite fictional character, or even as your alter-ego, in a no-judgment zone.Williams, 34, is a single Black father of two from San Diego, California, who currently resides in Atlanta. He once attended The Art Institute of Atlanta, but soon put his college life on hold to follow his dream as an illustrator. For the past 16 years he has been a professional freelance illustrator for comics and children’s books. In addition, he creates his own original characters. “My life has become drawing…that’s all i do,” said Williams in an interview with The AFRO. “I’m pretty much drawing all the time. When I’m eating, when I’m sleep and when I’m spending time with my kids”Williams added: “I illustrate things that I am passionate about. It’s just me drawing what I feel is cool, or what I wish I could see.”Williams maintains a blog at marcusthevisual.tumblr.com. One of Williams’ works on his blog is a comic he created called “Black Panther & Storm Heritage.” While not officially sanctioned by Marvel, the owner the Black Panther intellectual property, Williams’ creates his own story where the two main characters, who have a child and get divorced in the licensed comic book, stay together.“Those writers made them get a divorce, so I wanted to create a fan fiction that portrayed them still together,” said Williams. “It’s just the imagery. The imagery of showing a super hero black family that is powerful.”Williams is currently working on a comic called “Super Natural,” and “Super-Natural Boy.” No date is set for Super-Natural Boy yet, but his newest comic book called, “Super Natural Book 1,” will be published on September 28.“She’s going to deal with the police, she’s going to deal with the government, she’s going to deal with the media,” said Williams. “She’s new to our culture here in America, but since she’s powerful she’s going to be able to handle those entities much differently. I want to speak to that frustration.”This will not be Williams first time at the Baltimore Comic-Con convention. He will be drawing cats for guests and fans as he’ll turn their cat into a “hero cat” based off his illustrations he does for creator and writer, Kyle Puttkamer, called “Hero Cats of Stellar City.” The book allows an extensive group of cats with abilities to save their city as each cat gives off a distinct personality. “There’s not a lot of cat comic books out there…but there is a huge, huge, huge eagerish market for them, said Williams.”Williams also said: “We really don’t have to sell cats. People walk by our stand and say “Omg, hero cats!” Fanatics love cats already…we really don’t have to work too hard.”For more on “Hero Cats Of Stellar City” go to herocatscomic.com. Williams’ official website is marcusthevisual.com Baltimore Comic-Con will take place September 25-27.last_img read more

Atari Movie Makers Plan to Raise 40 Million via Bushnell Token Sale

first_img Popular on Variety ×Actors Reveal Their Favorite Disney PrincessesSeveral actors, like Daisy Ridley, Awkwafina, Jeff Goldblum and Gina Rodriguez, reveal their favorite Disney princesses. Rapunzel, Mulan, Ariel,Tiana, Sleeping Beauty and Jasmine all got some love from the Disney stars.More VideosVolume 0%Press shift question mark to access a list of keyboard shortcutsKeyboard Shortcutsplay/pauseincrease volumedecrease volumeseek forwardsseek backwardstoggle captionstoggle fullscreenmute/unmuteseek to %SPACE↑↓→←cfm0-9Next UpJennifer Lopez Shares How She Became a Mogul04:350.5x1x1.25×1.5x2xLive00:0002:1502:15 The makers of the upcoming Atari movie about the iconic video game console maker are eschewing traditional film financing models for a blockchain-powered initial coin offering (ICO), and plan to sell coins named after Atari founder Nolan Bushnell via a private pre-sale this spring. Film production and financing company Vision Tree plans to raise as much as $40 million with the sale of these Bushnell tokens.“While the film has received offers from conventional and studio-sourced financing, we have elected cryptocurrency funding to accelerate the filmmaking path in a whole new way, offering Atari fans the opportunity to share in the creation of this movie with us,” producer J.D. Seraphine told Variety.Owners of Bushnell tokens will get a share of the earnings of the movie, and they will also be able to vote on the trailer, and even help select cast members. “We wanted to enable Atari fans and gamers to co-own the project and the experience,” said Seraphine. “This open approach is also more in the DNA and the same spirit of Atari.”center_img This isn’t the first time that filmmakers are jumping onto the blockchain bandwagon. Earlier this week, Variety was first to report that the indie movie “No Postage Necessary” will use a blockchain-based app for promotion and distribution when it is being released this June.Seraphine told Variety that he has high hopes for the technology. “Blockchain technology and cryptocurrency financing will enable independent filmmakers and artists to not only create, but also own their projects that they can then grow over the span of their own career,” he said.The Atari movie is currently in pre-production, and is being produced by Leonardo DiCaprio’s production company Appian Way as well as Vision Tree and Avery Productions. It’s being pitched as a biopic about Atari’s founder and his life story of starting out as a pinball machine repairman who went on to found one of the most iconic video game console brands.Bushnell’s time at Atari recently came under scrutiny after allegations surfaced that he fostered a sexist culture at Atari. Bushnell was set to receive an award at the Game Developers Conference in San Francisco this week, but the conference rescinded the award in response to these allegations.At the time, Bushnell applauded the conference’s decision and apologized for any past indiscretions. In a statement sent to Variety this week, Bushnell chose to instead paint a different picture of Atari:“We created some extremely strong and capable professional women at Atari. Equal pay for equal work. Promoting women due to capability, and gender not being a promotional construct. We elevated women’s rights to the pinnacle of that time, and I’d do it all again just the same way.”last_img read more

Nanoscopic protein motion on a live cell membrane

first_img LEFT: (a) A TEM (transmission electron microscope) image of a filopodium including an EGFR–GNP. (b), A filopodium surface reconstructed from 780,000 trajectory points with a localization error of σx,y = 2 nm recorded at 1,000 fps. Inset, cross-sectional slice that depicts a cylindrical surface of diameter 150 nm after accounting for the size of the GNP. (c), A raw 13 min trajectory (left) broken into four subsequent pieces that reveal the journey to and from the tip, with arrows marking direction of net motion. (d), An ATOM plot of c, corrected for filopodium drift. (e), A surface interpolation from the final 80 s. The ring-like confinement in the final phase (marked with a triangle) is a 3D pit. The scale bars are 200 nm (a), 1 μm (x, y) and 200 nm (z) (b), 1,000 nm (c) and 100 nm (x, y) and 50 nm (z) (e). RIGHT: (a), A lateral trajectory of a 48 nm GNP probe. Scale bar, 100 nm. A lower temporal sampling of this confinement would have underestimated the extent of bounding. (b), Ci of the trajectory (using a time lag of five frames), which shows partially hindered diffusion with a propensity for freer diffusion in the centre. (c), An ATOM plot of a. (d), A cut through the 3D-ATOM plot along the line of the black triangle in c shows that occupancy favours an innermost disk-like region. The axes denote 100 nm in both c and d. (e), Conversion of the temporal 2D occupation from c into an effective potential energy distribution. (f–j), Equivalent to a–e, but for a 20 nm GNP probe. Credit: Nature Photonics, doi: 10.1038/s41566-019-0414-6 More information: Richard W. Taylor et al. Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane, Nature Photonics (2019). DOI: 10.1038/s41566-019-0414-6 Philipp Kukura et al. High-speed nanoscopic tracking of the position and orientation of a single virus, Nature Methods (2009). DOI: 10.1038/nmeth.1395 Jordan A. Krall et al. High- and Low-Affinity Epidermal Growth Factor Receptor-Ligand Interactions Activate Distinct Signaling Pathways, PLoS ONE (2011). DOI: 10.1371/journal.pone.0015945 In a recent study, Richard W. Taylor and colleagues at the interdisciplinary departments of Physics and Biology in Germany developed a new image processing approach to overcome this difficulty. They used the method to track the transmembrane epidermal growth factor receptor (EGFR) with nanometer scale precision in three dimensions (3-D). The technique allowed imaging across microseconds to minutes. The scientists provided examples of nanoscale motion and confinement using the method to image ubiquitous processes such as diffusion in plasma membranes, transport in filopodia and rotational motion during endocytosis. The results are now published in Nature Photonics. While steady progress in fluorescence microscopy has allowed scientists to monitor cellular events at the nanometer scale, a great deal still remains to be accomplished with advanced imaging systems. The challenges of fluorescence microscopy occurred due to the finite emission rate of a fluorescent source (dye molecule or semiconductor quantum dot), where too few photon emissions during a very small time-frame prevented effective or prolonged imaging. The central difficulty of scattering-based microscopy is relative to the nanoscopic probe, which competes against the background noise and a low signal-to-noise ratio (SNR); limiting the potential of imaging to only a few nanometers in high speed tracking experiments. iSCAT microscopy on live cells. a, Experimental arrangement of the iSCAT microscope for live-cell imaging. Cells are plated in a glass-bottomed dish under Leibowitz medium. (a) micropipette delivers the EGF–GNP probes directly onto the cell culture, where they specifically target the EGFR protein in the cell membrane. The bright-field illumination channel from above assists in inspecting the culture but is not required for iSCAT imaging. L1–L3, lenses; O1, ×100 objective; BS, 90:10 beam splitter; DM, 590 nm short-pass dichroic mirror. iSCAT imaging was performed with illumination intensities of 1–8 kW cm−2, which are known to be viable for HeLa at the wavelength of interest. Inset, wavefronts of the fields contributing to the iSCAT signal. (b), A section of the membrane of the HeLa cell before labelling, viewed via reflection iSCAT. (c), iSCAT image of the cell membrane including a bound EGF–GNP probe. (d), The PSF extracted from c. Scale bars in b–d are 1 μm. Credit: Nature Photonics, doi: 10.1038/s41566-019-0414-6 In the experiments, Taylor et al. introduced the epidermal growth factor-gold nanoparticle (EGF-GNP) probes to the sample chamber of the microscope using a micropipette to label the EGFRs (epidermal growth factor receptors) on HeLa cells and verified that the probes stimulated the EGFRs. Previous studies had already indicated that the probe size could influence rates of lipid diffusion in synthetic membranes, although they did not affect the mode of diffusion. Additionally, in live cells, molecular crowding was negligible for particles equal to or smaller than 50 nm. Diffusion on a filopodium. Credit: Nature Photonics, doi: 10.1038/s41566-019-0414-6 Journal information: Nature Photonics Taylor et al. verified these two concrete cases in the present work by comparing GNPs of varying diameters at 48 nm and 20 nm. The scientists then conducted fluorescent and biochemical studies to suggest that the EGF-coated GNPs activated EGFR signaling, much like the freely available EGFs, indicating that the label did not hinder biological functions. To overcome background noise related to molecular imaging the scientists implemented a new algorithm, which extracted the full iSCAT-point spread function (iSCAT-PSF) directly from each frame for clarity. Since existing techniques are unable to visualize features at high spatial and temporal resolution, many details on intracellular activity remain a matter of debate. In response, the new method by Taylor et al. offered a wealth of dynamic heterogeneities in 3-D to shed light on intracellular protein motion.The scientists first quantitatively studied subdiffusion in the plasma membrane by considering a 2-D example of the EGFR journey on the membrane of a living HeLa cell. For this, they computed the mean square displacement (MSD) for the whole trajectory of motion. Taylor et al. did not need to make assumptions on the nature of diffusion or its geographic landscape during the computation. They gauged the occurrence of biological diffractive barriers and confinements by observing the degree of directional correlation between two vectorial steps across a time span. Explore further Raw video of an epidermal growth factor-gold nanoparticle (EGFR–GNP) diffusing on a HeLa cell membrane. Credit: Nature Photonics, doi: 10.1038/s41566-019-0414-6 © 2019 Science X Network , Nature Methods The scientists thus gained insight on the nanoscopic details of diffusion along the filopodium and recorded the data across 13 minutes. They analyzed the 3-D trajectory to create the filopodium topography using gold nanoparticles as a ‘nano rover’ and mapped the surface topology of cellular structures for deeper examination. They plotted the trajectory ATOM (accumulated temporal occupancy map) and found that the 3-D representation was consistent with the biological step of pre-endocytic membrane invagination. High-speed microscopy techniques such as iSCAT are necessary to obtain high-resolution temporal information and prevent blurring effects during nanoparticle localization-based imaging. The scientists demonstrated this feature by recording confined diffusion at 30,000 fps (frames per second) with 48 nm and 20 nm GNPs. They followed the experiments with ultra-high-speed 3-D tracking of proteins at 66,000 fps using a short exposure time of 10 µs within a time duration of 3.5 seconds. Fast iSCAT microscopy imaging provided further evidence to reveal the intricate features of endocytic events relative to clathrin-mediated endocytosis in HeLa cells when simulated by low concentrations of EGF. In this way, Taylor et al. noted that the new technique could faithfully record nano-topographical information. The results matched the observations recorded with transmission electron microscopy (TEM) without significant differences on probe size reduction from 48 nm to 20 nm, while providing new insights. The new insights included details of subdiffusion, nanoscopic confinement, 3-D contours of filopodia and clathrin structures at the nanoscale. The scientists intend to combine iSCAT with in situ super-resolution fluorescence microscopy to understand the trajectories of proteins, viruses and other nanoscopic biological entities. Taylor et al. aim to advance the methods of image analysis to track GNPs smaller than 20 nm in the future and believe the new technology and additional optimization will allow them to specifically understand the life cycle of viruses without using an external label for tracking. In the present work, Taylor et al. used interferometric scattering (iSCAT) microscopy to track protein in live cell membranes. The method could visualize probe-cell interactions to understand the dynamics between diffusion and local topology. During the experiments, the scientists used gold nanoparticles (GNPs) to label epidermal growth factor receptors (EGFRs) in HeLa cells. The EGFRs are type I transmembrane proteins that can sense and respond to extracellular signals, whose aberrant signaling is linked to a variety of disease. Taylor et al. showed the GNP-labelled protein as a ‘nano-rover’ that mapped the nano-topology of cellular features such as membrane terrains, filopodia and clathrin structures. They provided examples of subdiffusion and nanoscopic confinement motion of a protein in 3-D at high temporal resolution and long time-points. Cellular functions are dictated by the intricate motion of proteins in membranes that span across a scale of nanometers to micrometers, within a time-frame of microseconds to minutes. However, this rich parameter of space is inaccessible using fluorescence microscopy, although it is within reach of interferometric scattering (iSCAT) particle tracking. The new iSCAT technique is, however, highly sensitive to single and unlabelled proteins, thereby causing non-specific background staining as a substantial challenge during cellular imaging. , PLoS ONE Citation: Nanoscopic protein motion on a live cell membrane (2019, May 22) retrieved 18 August 2019 from https://phys.org/news/2019-05-nanoscopic-protein-motion-cell-membrane.html Diffusion on the plasma membrane. (a), A lateral diffusional trajectory (17.5 μs exposure time, see color scale for chronology). (b), MSD (mean square displacement) versus τ. The blue curve shows the MSD of a. The black curve is simulated normal diffusion (α= 1), with the grey envelope indicating the uncertainty. (c), The diffusional exponent of rolling windows (color scale) over the trajectory. Regions of subdiffusion (α<1) are indicated by darker shades. (d), αi through time. The grey shading represents a mean uncertainty of 7 ± 4%, corresponding to a 95% confidence interval for a window of 100 ms (1,000 frames) and τ= 250 μs. The points marked with the asterisk correspond to the circle in c. (e), The step-direction Ci for rolling windows along the trajectory. (f), The step-direction Ci plotted through time, with the shading denoting uncertainty. (g), ATOM occupation plot with residency time (colour scale). The bin size corresponds to the localization error. Noteworthy regions of extended occupation, marked as loops and whirls (i)–(iii), are indicative of persistent nanoscopic structures. The enclosed region represents a dense patch of notable subdiffusion. Scale bars, 100 nm. Credit: Nature Photonics, doi: 10.1038/s41566-019-0414-6 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Nanoscale magnetic imaging of ferritin in a single cell The scientists then assessed the popularity of each trajectory pixel in space by introducing an accumulated temporal occupancy map (ATOM). In this technique, they divided the lateral plane of the trajectory into nanometer-sized bins and counted the occurrence of the particle in each bin. The results indicated the arrangement of nanostructures in loops and whirls within a minimal lifetime of 250 nanoseconds (5000 frames) to potentially portray a pre-endocytic step. In total, the simulated observations showed how protein diffusion was affected by the substructure of the cell.The iSCAT microscopy technique allowed scientists to record effects for a very long period of time, which they used together with 3-D imaging capabilities to follow EGFRs on a filopodium. The filopodia are biologically rod-like cellular protrusions containing bundles of actin filaments of up to 100 to 300 nm in diameter and 100 µm in length. The nanostructures can sense mechanical stimuli for chemoattraction or repulsion in the cellular microenvironment while providing sites for cell attachment. Ligand binding and EFGR activation on filopodia occurred at low concentrations of EGF, followed by its association with actin filaments and retrograde transport of EFGR to the cell body. last_img read more